Fluorescence detection.



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Fluorescence detection.

Fluorescence was recorded directly from protein samples as a function of X-ray energy using a photo-multiplier tube (Thorn EMI 9826B) couple to a cylinder of plastic scintillator material (NE technology Pilot U). Both PMT and scintillator are contained in a light tight aluminium housing and the front window is light insulated by aluminium foil.

The detector is mounted so that it accepts a solid angle of fluorescent radiation centred at to the incident beam in the horizontal plane. In this way the amount of scattered radiation entering the detector is minimised since the majority of the incident radiation is horizontally polarised as established by the use of the photo-emission device.



Gwyndaf Evans
Fri Oct 7 15:42:16 MET 1994